Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum.
Identifieur interne : 002610 ( Main/Exploration ); précédent : 002609; suivant : 002611Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum.
Auteurs : Jessica L. Linville [États-Unis] ; Miguel Rodriguez [États-Unis] ; Jonathan R. Mielenz [États-Unis] ; Chris D. Cox [États-Unis]Source :
- Bioresource technology [ 1873-2976 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Clostridium thermocellum, Populus.
- Cinétique, Fermentation, Hydrolyse.
English descriptors
- KwdEn :
- MESH :
- metabolism : Clostridium thermocellum, Populus.
- Fermentation, Hydrolysis, Kinetics.
Abstract
The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a higher growth rate (μmax=1.223 vs. 0.571 h(-1)) and less inhibition (KI,gen=0.991 vs. 0.757) in 10% v/v Populus hydrolysate compared to the WT. In 17.5% v/v Populus hydrolysate inhibition of PM increased slightly (KI,gen=0.888), whereas the WT was strongly inhibited and did not grow in a reproducible manner. Of the individual inhibitors tested, 4-hydroxybenzoic acid was the most inhibitory, followed by galacturonic acid. The PM did not have a greater ability to detoxify the hydrolysate than the WT.
DOI: 10.1016/j.biortech.2013.08.086
PubMed: 24036527
Affiliations:
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Linville</name><affiliation wicri:level="2"><nlm:affiliation>Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Rodriguez, Miguel" sort="Rodriguez, Miguel" uniqKey="Rodriguez M" first="Miguel" last="Rodriguez">Miguel Rodriguez</name><affiliation wicri:level="2"><nlm:affiliation>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Mielenz, Jonathan R" sort="Mielenz, Jonathan R" uniqKey="Mielenz J" first="Jonathan R" last="Mielenz">Jonathan R. Mielenz</name><affiliation wicri:level="2"><nlm:affiliation>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Cox, Chris D" sort="Cox, Chris D" uniqKey="Cox C" first="Chris D" last="Cox">Chris D. Cox</name><affiliation wicri:level="2"><nlm:affiliation>Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN, United States. 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A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a higher growth rate (μmax=1.223 vs. 0.571 h(-1)) and less inhibition (KI,gen=0.991 vs. 0.757) in 10% v/v Populus hydrolysate compared to the WT. In 17.5% v/v Populus hydrolysate inhibition of PM increased slightly (KI,gen=0.888), whereas the WT was strongly inhibited and did not grow in a reproducible manner. Of the individual inhibitors tested, 4-hydroxybenzoic acid was the most inhibitory, followed by galacturonic acid. The PM did not have a greater ability to detoxify the hydrolysate than the WT.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24036527</PMID><DateCompleted><Year>2014</Year><Month>04</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>147</Volume><PubDate><Year>2013</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum.</ArticleTitle><Pagination><MedlinePgn>605-613</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(13)01316-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2013.08.086</ELocationID><Abstract><AbstractText>The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a higher growth rate (μmax=1.223 vs. 0.571 h(-1)) and less inhibition (KI,gen=0.991 vs. 0.757) in 10% v/v Populus hydrolysate compared to the WT. In 17.5% v/v Populus hydrolysate inhibition of PM increased slightly (KI,gen=0.888), whereas the WT was strongly inhibited and did not grow in a reproducible manner. Of the individual inhibitors tested, 4-hydroxybenzoic acid was the most inhibitory, followed by galacturonic acid. The PM did not have a greater ability to detoxify the hydrolysate than the WT.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Linville</LastName><ForeName>Jessica L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodriguez</LastName><ForeName>Miguel</ForeName><Initials>M</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mielenz</LastName><ForeName>Jonathan R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cox</LastName><ForeName>Chris D</ForeName><Initials>CD</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, United States; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN, United States. Electronic address: ccox9@utk.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D048013" MajorTopicYN="N">Clostridium thermocellum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biofuels</Keyword><Keyword MajorTopicYN="N">Clostridium thermocellum</Keyword><Keyword MajorTopicYN="N">Inhibition</Keyword><Keyword MajorTopicYN="N">Kinetic model</Keyword><Keyword MajorTopicYN="N">Populus hydrolysate</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24036527</ArticleId><ArticleId IdType="pii">S0960-8524(13)01316-3</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2013.08.086</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Linville, Jessica L" sort="Linville, Jessica L" uniqKey="Linville J" first="Jessica L" last="Linville">Jessica L. Linville</name></region><name sortKey="Cox, Chris D" sort="Cox, Chris D" uniqKey="Cox C" first="Chris D" last="Cox">Chris D. Cox</name><name sortKey="Mielenz, Jonathan R" sort="Mielenz, Jonathan R" uniqKey="Mielenz J" first="Jonathan R" last="Mielenz">Jonathan R. Mielenz</name><name sortKey="Rodriguez, Miguel" sort="Rodriguez, Miguel" uniqKey="Rodriguez M" first="Miguel" last="Rodriguez">Miguel Rodriguez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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